ISO INTERNATIONAL STANDARD 23893-3 First edition 2013-04-01 Water quality Biochemical and physiological measurements on fish - Part 3: Determination of vitellogenin Qualite de I'eau - Mesurages biochimiques et physiologiques sur poisson- Partie 3: Dosage de la vitellogénine Reference number IS0 23893-3:2013(E) ISO @IS02013 nitted without license from IHS Not for Resale IS0 23893-3:2013(E) COPYRIGHTPROTECTEDDOCUMENT @IS02013 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax +41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ IS0 2013 - All rights reserved No reproduction or networking permited without license from IHS Not for Resale IS0 23893-3:2013(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Minimum performance criteria .2 6 Test environment 7 Reagents ..3 8 Apparatus ..3 9 Sampling procedure ..4 9.1 Sampling of fish 4 9.2 Sampling of blood plasma 4 9.3 Storage of blood plasma samples ..5 10 Analytical procedure. ..5 10.1 Preparation of the samples .5 10.2 Determination ofvitellogenin ..5 11 Test report ..10 Annex A (informative) Examples of results: Fathead minnow sandwich ELISA ..12 Bibliography .19 iii vithout license from IHS Not for Resale