ISO INTERNATIONAL STANDARD 6341 Fourth edition 2012-10-15 Water guality Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) Acute toxicity test Qualite de I'eau- Determination de Il'inhibition de la mobilite de Daphnia magna Straus (Cladocera, Crustacea) Essai de toxicite aigue Reference number ISO 6341:2012(E) ISO @ISO2012 IHS under itted without license from IHS Not for Resale ISO 6341:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISO 2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Casepostale56·CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 4122 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ ISO 2012 -All rights reserved No reproductic etworking permitted without license from IHS Not for Resale ISO 6341:2012(E) Contents Page Foreword ..iv Introduction 1 Scope 2 Normative references 3 Terms and definitions. 4 Principle 5 Test environment.. 2 6 Reagents, test organisms and media 3 7 Apparatus and materials. 8 Treatment and preparation of samples .. 8.1 Special precautions for sampling, transportation, storage and treatment of water, effluent, or aqueous extract samples to be tested... 5 8.2 Preparation of solutions of substances to be tested 9 Procedure 6 9.1 General.. 9.2 Preliminary test. 9.3 Definitive test.. 9.4 Check of the sensitivity of the Daphnia magna and conformity with the procedure 9.5 Limit test. 8 10 Interpretation and validity of the results 8 10.1 EstimationoftheEC50 .8 10.2 Validity criteria .. 8 11 Expression of results .8 12 Test report. AnnexA(informative)PreparationoftheElendtM4medium.. 10 Annex B (informative) Example of graphical determination of the inhibition of mobility of Daphnia Annex C (informative) General recommendations for stock culturing . .16 Annex D (informative) Culturing of Daphnia magna for production of dormant eggs .17 Annex E (informative) Precision data.. .19 Annex F (informative) Dilution level D - Preparation of the dilution series for the determination of the LID 20 Bibliography. 22 ili t license from IHS Not for Resale